                         SEQUENCE LISTING

<110>  Commonwealth Scientific and Industrial Research 
       Organisation
       Orica Australia Pty Ltd
 
<120>  Enzymes for degrading organophosphates

<130>  512323

<150>  US 61/509,810
<151>  2011-07-20

<150>  US 61/510,377
<151>  2011-07-21

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  356
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant OpdA enzyme (A900)

<400>  1

Pro Ile Gly Thr Gly Asp Leu Ile Asn Thr Val Arg Gly Pro Ile Pro 
1               5                   10                  15      


Val Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly Ser 
            20                  25                  30          


Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg Lys 
        35                  40                  45              


Ala Leu Val Glu Lys Ala Val Arg Gly Leu Arg His Ala Arg Ala Ala 
    50                  55                  60                  


Gly Val Gln Thr Ile Val Asp Val Ser Thr Phe Asp Ile Gly Arg Asp 
65                  70                  75                  80  


Val Arg Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile Val 
                85                  90                  95      


Ala Ala Thr Gly Leu Trp Phe Asp Pro Pro Leu Ser Met Arg Met Arg 
            100                 105                 110         


Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln His Gly 
        115                 120                 125             


Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr Thr 
    130                 135                 140                 


Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Arg Ala Ala Ala Arg 
145                 150                 155                 160 


Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ser Ala Ser 
                165                 170                 175     


Gln Arg Asp Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly Leu 
            180                 185                 190         


Ser Pro Ser Arg Val Cys Ile Gly His Ser Glu Asp Thr Asp Asp Leu 
        195                 200                 205             


Ser Tyr Leu Thr Gly Leu Ala Ala Arg Gly Tyr Leu Val Gly Leu Asp 
    210                 215                 220                 


Arg Met Pro Tyr Ser Ala Ile Gly Leu Glu Gly Asp Ala Ser Ala Leu 
225                 230                 235                 240 


Ala Leu Phe Gly Thr Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile Lys 
                245                 250                 255     


Ala Leu Ile Asp Arg Gly Tyr Lys Asp Arg Ile Leu Val Ser His Asp 
            260                 265                 270         


Trp Leu Phe Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val Met 
        275                 280                 285             


Asp Arg Ile Asn Pro Asp Gly Met Ala Phe Val Pro Leu Arg Val Ile 
    290                 295                 300                 


Pro Phe Leu Arg Glu Lys Gly Val Pro Pro Glu Thr Leu Ala Gly Val 
305                 310                 315                 320 


Thr Val Ala Asn Pro Ala Arg Phe Leu Ser Pro Thr Val Arg Ala Val 
                325                 330                 335     


Val Thr Arg Ser Glu Thr Ser Arg Pro Ala Ala Pro Ile Pro Arg Gln 
            340                 345                 350         


Asp Thr Glu Arg 
        355     


<210>  2
<211>  357
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Native OpdA without signal sequence and with N-terminal Met

<400>  2

Met Pro Ile Gly Thr Gly Asp Leu Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Pro Val Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Ala Glu Lys Ala Val Arg Gly Leu Arg His Ala Arg Ser 
    50                  55                  60                  


Ala Gly Val Gln Thr Ile Val Asp Val Ser Thr Phe Asp Ile Gly Arg 
65                  70                  75                  80  


Asp Val Arg Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Phe Asp Pro Pro Leu Ser Met Arg Met 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln His 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Lys Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ser Ala 
                165                 170                 175     


Ser Gln Arg Asp Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Gly Leu Ala Ala Arg Gly Tyr Leu Val Gly Leu 
    210                 215                 220                 


Asp Arg Met Pro Tyr Ser Ala Ile Gly Leu Glu Gly Asn Ala Ser Ala 
225                 230                 235                 240 


Leu Ala Leu Phe Gly Thr Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Arg Gly Tyr Lys Asp Arg Ile Leu Val Ser His 
            260                 265                 270         


Asp Trp Leu Phe Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Arg Ile Asn Pro Asp Gly Met Ala Phe Val Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Pro Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Val Thr Val Ala Asn Pro Ala Arg Phe Leu Ser Pro Thr Val Arg Ala 
                325                 330                 335     


Val Val Thr Arg Ser Glu Thr Ser Arg Pro Ala Ala Pro Ile Pro Arg 
            340                 345                 350         


Gln Asp Thr Glu Arg 
        355         


<210>  3
<211>  357
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant OpdA enzyme (M4)

<400>  3

Met Pro Ile Gly Thr Gly Asp Leu Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Pro Val Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Ala Glu Lys Ala Val Arg Gly Leu Arg His Ala Arg Ser 
    50                  55                  60                  


Ala Gly Val Gln Thr Ile Val Asp Val Ser Thr Phe Asp Ile Gly Arg 
65                  70                  75                  80  


Asp Val Arg Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Phe Asp Pro Pro Leu Ser Met Arg Met 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln His 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Arg Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ser Ala 
                165                 170                 175     


Ser Gln Arg Gly Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Gly Leu Ala Ala Arg Gly Tyr Leu Val Gly Leu 
    210                 215                 220                 


Asp Arg Met Pro Tyr Ser Ala Ile Gly Leu Glu Gly Asp Ala Ser Ala 
225                 230                 235                 240 


Leu Ala Leu Phe Gly Asn Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Arg Gly Tyr Lys Asp Arg Ile Leu Val Ser His 
            260                 265                 270         


Asp Trp Leu Phe Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Arg Ile Asn Pro Asp Gly Met Ala Phe Val Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Pro Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Val Thr Val Ala Asn Pro Ala Arg Phe Leu Ser Pro Thr Val Arg Ala 
                325                 330                 335     


Val Val Thr Arg Ser Glu Thr Ser Arg Pro Ala Ala Pro Ile Pro Arg 
            340                 345                 350         


Gln Asp Thr Glu Arg 
        355         


<210>  4
<211>  333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OPH miunus signal sequence and with N-terminal Met

<400>  4

Met Gly Asp Arg Ile Asn Thr Val Arg Gly Pro Ile Thr Ile Ser Glu 
1               5                   10                  15      


Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly Ser Ser Ala Gly 
            20                  25                  30          


Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg Lys Ala Leu Ala 
        35                  40                  45              


Glu Lys Ala Val Arg Gly Leu Arg Arg Ala Arg Ala Ala Gly Val Arg 
    50                  55                  60                  


Thr Ile Val Asp Val Ser Thr Phe Asp Ile Gly Arg Asp Val Ser Leu 
65                  70                  75                  80  


Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile Val Ala Ala Thr 
                85                  90                  95      


Gly Leu Trp Phe Asp Pro Pro Leu Ser Met Arg Leu Arg Ser Val Glu 
            100                 105                 110         


Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln Tyr Gly Ile Glu Asp 
        115                 120                 125             


Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr Thr Gly Lys Ala 
    130                 135                 140                 


Thr Pro Phe Gln Glu Leu Val Leu Lys Ala Ala Ala Arg Ala Ser Leu 
145                 150                 155                 160 


Ala Thr Gly Val Pro Val Thr Thr His Thr Ala Ala Ser Gln Arg Asp 
                165                 170                 175     


Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly Leu Ser Pro Ser 
            180                 185                 190         


Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp Leu Ser Tyr Leu 
        195                 200                 205             


Thr Ala Leu Ala Ala Arg Gly Tyr Leu Ile Gly Leu Asp His Ile Pro 
    210                 215                 220                 


His Ser Ala Ile Gly Leu Glu Asp Asn Ala Ser Ala Ser Ala Leu Leu 
225                 230                 235                 240 


Gly Ile Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile Lys Ala Leu Ile 
                245                 250                 255     


Asp Gln Gly Tyr Met Lys Gln Ile Leu Val Ser Asn Asp Trp Leu Phe 
            260                 265                 270         


Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val Met Asp Arg Val 
        275                 280                 285             


Asn Pro Asp Gly Met Ala Phe Ile Pro Leu Arg Val Ile Pro Phe Leu 
    290                 295                 300                 


Arg Glu Lys Gly Val Pro Gln Glu Thr Leu Ala Gly Ile Thr Val Thr 
305                 310                 315                 320 


Asn Pro Ala Arg Phe Leu Ser Pro Thr Leu Arg Ala Ser 
                325                 330             


<210>  5
<211>  357
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A900 with N-terminal Met

<400>  5

Met Pro Ile Gly Thr Gly Asp Leu Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Pro Val Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Val Glu Lys Ala Val Arg Gly Leu Arg His Ala Arg Ala 
    50                  55                  60                  


Ala Gly Val Gln Thr Ile Val Asp Val Ser Thr Phe Asp Ile Gly Arg 
65                  70                  75                  80  


Asp Val Arg Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Phe Asp Pro Pro Leu Ser Met Arg Met 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln His 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Arg Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ser Ala 
                165                 170                 175     


Ser Gln Arg Asp Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Glu Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Gly Leu Ala Ala Arg Gly Tyr Leu Val Gly Leu 
    210                 215                 220                 


Asp Arg Met Pro Tyr Ser Ala Ile Gly Leu Glu Gly Asp Ala Ser Ala 
225                 230                 235                 240 


Leu Ala Leu Phe Gly Thr Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Arg Gly Tyr Lys Asp Arg Ile Leu Val Ser His 
            260                 265                 270         


Asp Trp Leu Phe Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Arg Ile Asn Pro Asp Gly Met Ala Phe Val Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Pro Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Val Thr Val Ala Asn Pro Ala Arg Phe Leu Ser Pro Thr Val Arg Ala 
                325                 330                 335     


Val Val Thr Arg Ser Glu Thr Ser Arg Pro Ala Ala Pro Ile Pro Arg 
            340                 345                 350         


Gln Asp Thr Glu Arg 
        355         


<210>  6
<211>  1071
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence for A900

<400>  6
ccaatcggta caggcgatct gattaatact gttcgcggcc ccattccagt ttcggaagcg       60

ggcttcacac tgacccatga gcatatctgc ggcagttcgg cgggattcct acgtgcgtgg      120

ccggagtttt tcggtagccg caaagctcta gttgaaaagg ctgtgagagg attacgccat      180

gccagagcgg ctggcgtgca aaccatcgtc gatgtgtcga ctttcgatat cggtcgtgac      240

gtccgtttat tggccgaagt ttcgcgggcc gccgacgtgc acatcgtggc ggcgactggc      300

ttatggttcg acccgccact ttcaatgcga atgcgcagcg tcgaagaact gacccagttc      360

ttcctgcgtg aaatccaaca tggcatcgaa gacaccggta ttagggcggg cattatcaag      420

gtcgcgacca cagggaaggc gacccccttt caagagttgg tgttacgtgc agccgcgcgg      480

gccagcttgg ccaccggtgt tccggtaacc actcacacgt cagcaagtca gcgcgatggc      540

gagcagcagg cagccatatt tgaatccgaa ggtttgagcc cctcacgggt ttgtatcggt      600

cacagcgaag atactgacga tttgagctac ctaaccggcc tcgctgcgcg cggatacctc      660

gtcggtttag atcgcatgcc gtacagtgcg attggtctag aaggcgatgc gagtgcatta      720

gcgctctttg gtactcggtc gtggcaaaca agggctctct tgatcaaggc gctcatcgac      780

cgaggctaca aggatcgaat cctcgtctcc catgactggc tgttcgggtt ttcgagctat      840

gtcacgaaca tcatggacgt aatggatcgc ataaacccag atggaatggc cttcgtccct      900

ctgagagtga tcccattcct acgagagaag ggcgtcccgc cggaaacgct agcaggcgta      960

accgtggcca atcccgcgcg gttcttgtcc ccgaccgtgc gggccgtcgt gacacgatct     1020

gaaacttccc gccctgccgc gcctattccc cgtcaagata ccgaacgatg a              1071


<210>  7
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TAT signal peptide

<400>  7

Met Ser Leu Ser Arg Arg Gln Phe Ile Gln Ala Ser Gly Ile Ala Leu 
1               5                   10                  15      


Cys Ala Gly Ala Val Pro Leu Lys Ala Ser Ala 
            20                  25          


